bet356ÌåÓýÔÚÏß

½Ó´ý»á¼ûbet356ÌåÓýÔÚÏß¹Ù·½ÍøÕ¾ £¡
bet356ÌåÓýÔÚÏß(ÑÇÖÞ°æ)¹Ù·½ÍøÕ¾-App Store

ÊÖÒÕÖ§³Ö

ÖÂÁ¦ÓÚÒý½øÍâÑóÏȽøÊÖÒÕ£¬Îª¿í´ó¿ÆÑÐÊÂÇéÕߺÍÒ½ÁÆ»ú¹¹Ìṩ¸ßÖÊÁ¿ µÄ¿ÆÑÐÊÖÒÕÖ§³Ö ¡£

¸ßͨÁ¿²âÐòÑùÆ·

ÑùÆ·ÖƱ¸Ö¸ÄÏ


¶¯Ö²Îï»ùÒò×éde novo ²âÐò

 

ÑùÆ·×ÜÁ¿£ºÐ¡Æ¬¶ÏÎÄ¿âÑù±¾ÖƱ¸ÐèÒª×ÜÁ¿´óÓÚ1 ¦Ìg µÄÑùÆ·£¬mate-pair ÎĿ⣨2 kb¡¢5 kb¡¢10 kb¡¢£©ÑùÆ·£¬ÖƱ¸ÐèÒª×ÜÁ¿20-50 ¦Ìg £¬

Ϊ°ü¹ÜʵÑéÖÊÁ¿¼°ÑÓÐøÐÔ£¬ÇëÒ»´ÎÐÔÌṩÖÁÉÙ2 ´ÎÑù±¾ÖƱ¸×ÜÁ¿;

ÑùƷŨ¶È´¿¶È£ºÑùƷŨ¶È>200 ng/¦Ìl£¬OD260/280 ½éÓÚ1.8-2.0 Ö®¼ä£¬ÎÞÂÑ°×ÖÊ¡¢RNA »òÕßÈâÑۿɼûÔÓÖÊÎÛȾ;

ÑùÆ·ÖÊÁ¿£º»ùÒò×éÍêÕû¡¢ÎÞ½µ½â£¬µçӾЧ¹û»ùÒò×éDNA Ö÷´øÓ¦ÔÚÈë-Hind llldigest ×î´óÌõ´ø23 KB ÒÔÉÏÇÒÖ÷´øÇåÎú£¬ÎÞÃÖÉ¢;

ÑùÆ·ÉúÑÄ£ºÇëÉúÑÄÓڸɷۡ¢¾Æ¾«¡¢TE buffer »ò³¬´¿Ë®ÖУ¬²¢ÇëÔÚÑùÆ·ÐÅÏ¢µ¥ÖÐ×¢Ã÷¡£

 

¶¯Ö²Îï»ùÒò×éÖزâÐò

 

DNA ÑùÆ·×ÜÁ¿£ÔÃĿν¨¿âÐèҪ׼±¸ÑùÆ·1 ¦Ìg£¬½¨Òéµ¥´ÎËÍÑùÌṩ2 ´ÎÖƱ¸×ÜÁ¿£»

ÑùƷŨ¶ÈºÍ´¿¶È£ºÑùƷŨ¶È>50 ng/¦Ìl£¬OD260/280 ½éÓÚ1.8-2.0 Ö®¼ä£¬ÎÞÈâÑۿɼûÔÓÖÊÎÛȾ£¬»ùÒò×éÍêÕû¡¢ÎÞ½µ½â£¬µçÓ¾ÖÐDNA Ö÷´ø

Ó¦´óÓÚ23 kb£»

ÑùÆ·Ñ¡Ôñ£º¹ØÓÚÖ²ÎïÑùÆ·½¨ÒéÑ¡È¡ÆáºÚ×÷ÓýµÄ»Æ»¯Ãç»òÄÛÃ磬¶¯ÎïÑùƷӦѡÔñ¼¡È⡢ѪµÈÖ¬·¾º¬Á¿½ÏÉÙµÄ×éÖ¯¾ÙÐÐÈ¡Ñù£»

ÑùÆ·ÉúÑÄ£ºÇëÑ¡Ôñ¸É·Û¡¢¾Æ¾«¡¢TE »ò³¬´¿Ë®µÄÉúÑÄ·½·¨¡£

 

È«ÍâÏÔ×Ó×é¼°Ä¿µÄÇøÓòÖزâÐò

 

DNA ÑùÆ·×ÜÁ¿£ºÇëÒ»´ÎÐÔÌṩÖÁÉÙ1 ¦Ìg ¸ßÖÊÁ¿µÄ»ùÒò×éDNA£»

ÑùƷŨ¶ÈºÍ´¿¶È£ºÑùƷŨ¶È>50 ng/¦Ìl£¬OD260/280 ½éÓÚ1.8-2.0 Ö®¼ä£¬ÎÞÈâÑۿɼûÎÛȾ;

ÑùÆ·ÖÊÁ¿£º»ùÒò×éÍêÕû¡¢ÎÞ½µ½â£¬µçÓ¾ÖÐDNA Ö÷´øÓ¦´óÓÚ23 kb£»

ÑùÆ·Ñ¡Ôñ£º¹ØÓÚÖ²ÎïÑùÆ·½¨ÒéÑ¡È¡ÆáºÚ×÷ÓýµÄ»Æ»¯Ãç»òÄÛÃ磬¶¯ÎïÑùƷӦѡÔñ¼¡È⡢ѪµÈÖ¬·¾º¬Á¿½ÏÉÙµÄ×éÖ¯¾ÙÐÐÈ¡Ñù;

ÑùÆ·ÉúÑÄ£ºÇëÑ¡Ôñ¸É·Û¡¢¾Æ¾«¡¢TE »ò³¬´¿Ë®ÖоÙÐÐÉúÑÄ¡£

 

΢ÉúÎï»ùÒò×éde novo ²âÐò

 

DNA ÑùÆ·×ÜÁ¿£ºÐ¡Æ¬¶ÏÎÄ¿âÑùÆ·ÖƱ¸ÐèÒª×ÜÁ¿3 ¦Ìg µÄÑùÆ·£¬mate-pair ÎĿ⣨2 kb¡¢5 kb¡¢10 kb£©ÑùÆ·ÖƱ¸ÐèÒª10-50 ¦Ìg µÄÑù

Æ·, Ϊ°ü¹ÜʵÑéÖÊÁ¿¼°ÑÓÐøÐÔ£¬ÇëÒ»´ÎÐÔÌṩÖÁÉÙ2 ´ÎÑùÆ·ÖƱ¸Á¿;

ÑùƷŨ¶ÈºÍ´¿¶È£ºÑùƷŨ¶È>100 ng/¦Ìl£¬OD260/280 ½éÓÚ1.8-2.0 Ö®¼ä£¬ÎÞÈâÑۿɼûÎÛȾ;

ÑùÆ·ÖÊÁ¿£º»ùÒò×éÍêÕû¡¢ÎÞ½µ½â;

ÑùÆ·Ñ¡Ôñ£º¹ØÓÚϸ¾úºÍÕæ¾úÑùÆ·£¬Ï£ÍûÖ»¹ÜÀ´×ÔÓÚͳһ¸ö¾úÖ꣬²»ÒªÓÐËÞÖ÷DNA ÎÛȾ;

ÑùÆ·ÉúÑÄ£ºÇëÑ¡Ôñ¸É·Û¡¢¾Æ¾«¡¢TE »ò³¬´¿Ë®µÄÉúÑÄ·½·¨Ö®Ò»¡£

 

ת¼×é²âÐò


ת¼×éÑùÆ·£ºRNA ÑùÆ·×ÜÁ¿: ¡Ý 1.0 ¦Ìg£¬RNA ÑùƷŨ¶È: ¡Ý 50 ng/¦Ìl£»

ÑùƷŨ¶ÈºÍ´¿¶È£ºÑùƷŨ¶È>100 ng/¦Ìl£¬OD260/280 ½éÓÚ1.8-2.0 Ö®¼ä£¬ÎÞÈâÑۿɼûÎÛȾ;

ÑùÆ·ÖÊÁ¿£ºÊ¹ÓÃAgilent Bioanalyzer ÒÇÆ÷¼ì²â£¬Ôò»ñµÃµÄ×ܵÄRNA 28S£º18S >1£¬RIN ¶¯Îï>7£¬RIN Ö²Îï >6.5£»

ÑùÆ·Ñ¡Ôñ£º¹ØÓÚϸ¾úºÍÕæ¾úÑùÆ·£¬Ï£ÍûÖ»¹ÜÀ´×ÔÓÚͳһ¸ö¾úÖ꣬²»ÒªÓÐËÞÖ÷DNA ÎÛȾ;

ÑùÆ·ÉúÑÄ£ºÇëÑ¡ÔñÒÒ´¼¡¢DEPC Ë®ÉúÑÄÑùÆ·£¬²¢ÔÚÑùÆ·ÐÅÏ¢µ¥ÖÐ×¢Ã÷;

ÑùÆ·ÔËÊ䣺ÑùÆ·ÇëÖÃÓÚ1.5 ml ¹ÜÖУ¬²¢Ê¹Ó÷â¿ÚĤ·âºÃºó¸É±ùÔËÊä, ÇëÎðÖظ´¶³ÈÚ¡£


СRNA ²âÐò


RNA ÑùÆ·×ÜÁ¿£ºÒ»´ÎÐÔÌṩÖÁÉÙ3 ¦Ìg ¸ßÖÊÁ¿µÄ×ÜRNA£»

ÑùƷŨ¶ÈºÍ´¿¶È£ºÑùƷŨ¶È>100 ng/¦Ìl£¬OD260/280 ½éÓÚ1.8-2.0 Ö®¼ä£¬ÎÞÈâÑۿɼûÎÛȾ;

ÑùÆ·ÖÊÁ¿£ºÊ¹ÓÃAgilent Bioanalyzer ÒÇÆ÷¼ì²â£¬Ôò»ñµÃµÄ×ܵÄRNA 28S£º18S >1£¬RNA>7.5£»

ÑùÆ·ÉúÑÄ£ºÇëÑ¡ÔñÒÒ´¼¡¢DEPC Ë®ÉúÑÄÑùÆ·£¬²¢ÔÚÑùÆ·ÐÅÏ¢µ¥ÖÐ×¢Ã÷;

ÑùÆ·ÔËÊ䣺ÑùÆ·ÇëÖÃÓÚ1.5 ml ¹ÜÖУ¬²¢Ê¹Ó÷â¿ÚĤ·âºÃ£¬°²ÅÅ×ã¹»µÄ¸É±ùÔËÊä¡£

 

lncRNA ²âÐò

 

ת¼×éÑùÆ·£ºRNA ÑùÆ·×ÜÁ¿ ¡Ý 2.0 ¦Ìg£¬RNA ÑùƷŨ¶È ¡Ý 100 ng/¦Ìl£»

ÑùƷŨ¶ÈºÍ´¿¶È£ºÑùƷŨ¶È>100 ng/¦Ìl£¬OD260/280 ½éÓÚ1.8-2.0 Ö®¼ä£¬ÎÞÈâÑۿɼûÎÛȾ;

ÑùÆ·ÖÊÁ¿£ºÊ¹ÓÃAgilent Bioanalyzer ÒÇÆ÷¼ì²â£¬Ôò»ñµÃµÄ×ܵÄRNA 28S£º18S>1£¬RIN Öµ >7.5£»

ÑùÆ·Ñ¡Ôñ£º¹ØÓÚϸ¾úºÍÕæ¾úÑùÆ·£¬Ï£ÍûÖ»¹ÜÀ´×ÔÓÚͳһ¸ö¾úÖ꣬²»ÒªÓÐËÞÖ÷DNA ÎÛȾ;

ÑùÆ·ÉúÑÄ£ºÇëÑ¡ÔñÒÒ´¼¡¢DEPC Ë®ÉúÑÄÑùÆ·£¬²¢ÔÚÑùÆ·ÐÅÏ¢µ¥ÖÐ×¢Ã÷;

ÑùÆ·ÔËÊ䣺ÑùÆ·ÇëÖÃÓÚ1.5 ml ¹ÜÖУ¬²¢Ê¹Ó÷â¿ÚĤ·âºÃºó¸É±ùÔËÊ䣬ÇëÎðÖظ´¶³ÈÚ¡£

 

±í¹ÛÒÅ´«Ñ§Ïà¹ØDNA ²âÐò

 

DNA ÑùÆ·×ÜÁ¿£ºÈ«»ùÒò¼×»ù»¯²âÐòÐèÌṩ3 ¦Ìg µÄ»ùÒò×éDNA£¬RRBS ÐèÌṩÖÁÉÙ4 ¦Ìg µÄDNA£¬ChIP-seq ºÍMeIDP-seq ÐèÌṩ

ÖÁÉÙ30 ng µÄ¿¹Ì帻¼¯ºóµÄDNA Ƭ¶ÏÑùÆ·£¨Æ¬¶Ï³¤¶È200-500 bp£©£¬Îª°ü¹ÜÖÊÁ¿ºÍÑÓÐøÐÔ£¬ÇëÒ»´ÎÐÔÌṩÖÁÉÙ2 ´ÎÑù±¾ÖƱ¸Á¿£»

ÑùƷŨ¶ÈºÍ´¿¶È£º»ùÒò×éDNA ÑùƷŨ¶È>100 ng/¦Ìl£¬¸»¼¯ºóDNA ÑùƷŨ¶È>5 ng/¦Ìl£¬OD260/280 ½éÓÚ1.8-2.0 Ö®¼ä£¬ÎÞÈâÑۿɼûÎÛȾ£»

ÑùÆ·ÉúÑÄ£ºÇëÑ¡ÔñÒÒ´¼¡¢´¿Ë®¾ÙÐÐÉúÑÄ£¬²¢ÔÚÑùÆ·ÐÅÏ¢µ¥ÖÐ×¢Ã÷£»

ÑùÆ·ÔËÊ䣺Ç뽫ÑùÆ·ÖÃÓÚ1.5 ml ¹ÜÖУ¬²¢Ó÷â¿ÚĤ·âºÃ¡£

 

ºê»ùÒò×é²âÐò

 

ÑùÆ·×ÜÁ¿£ºÇéÐÎϸ¾ú¶àÑùÐÔ·ÖÀàÒ»´ÎÎÄ¿âÖƱ¸ËùÐèÒªµÄÑùÆ·Á¿Îª1 ¦Ìg DNA£¬ºê»ùÒò×é²âÐòÒ»´ÎÎÄ¿âÖƱ¸ËùÐèÒªµÄÑùÆ·Á¿Îª3 ¦Ìg DNA£¬

Ϊ°ü¹ÜʵÑéµÄÖÊÁ¿¼°ÑÓÐøÐÔ£¬ÇëÒ»´ÎÐÔÌṩÖÁÉÙÁ½´ÎµÄÖƱ¸Á¿£¨×¢£º²î±ðÇéÐÎDNA ÖÊÁ¿²î±ð½Ï´ó£¬ÏêϸÑùÆ·Á¿Çë×ÉѯÍâµØÊÖÒÕÖ°Ô±£©£»

ÑùƷŨ¶ÈºÍ´¿¶È£ºÇéÐÎÑùƷŨ¶È>50 ng/¦Ìl£¬ºê»ùÒò×éΪ30 ng/¦Ìl£¬OD260/280 ½éÓÚ1.8-2.0 Ö®¼ä£¬ÎÞÂÑ°×ÖÊ¡¢RNA »òÈâÑۿɼûÔÓÖÊÎÛȾ£»

ÑùÆ·ÖÊÁ¿£º»ùÒò×éÍêÕû¡¢ÎÞ½µ½â£¬µçӾЧ¹û»ùÒò×éDNA Ö÷´øÔÚÔ¤ÆÚλÖã¬ÇåÎú£¬ÎÞÃÖÉ¢£¬cDNA Ƭ¶Ï»¯ÑùÆ·£¬Æ¬¶Ï¾ÞϸÇкÏÔ¤ÆÚλÖã»

ÑùÆ·ÉúÑÄ£ºÇëÑ¡Ôñ¸É·Û¡¢¾Æ¾«¡¢TE buffer »ò³¬´¿Ë®Ò»ÖÖ£¬²¢ÔÚÑùÆ·ÐÅÏ¢µ¥ÖÐ×¢Ã÷£»

ÑùÆ·ÔËÊ䣺Ç뽫ÑùÆ·ÖÃÓÚ1.5 ml ¹ÜÖУ¬×öºÃ±ê¼Ç£¬²¢Ó÷â¿ÚĤºÃ£¬²¢Í¬¡¶²âÐòÑùÆ·¶©µ¥¡·Ò»Æð·ÅÔÚËÜÁÏÑùÆ·´ü·â¿Ú£¬»ùÒò×éDNA ÈôÊÇ

ÓÃÒÒ´¼³Áµí£¬¿ÉÒÔ³£ÎÂÔËÊä¡£

 

˫øÇÐRAD ²âÐò

 

¿Í»§Ìṩ£ºÄ¿µÄÎïÖֵĻùÒò×éÐÅÏ¢»ò½üÔ´²Î¿¼»ùÒò×éµÄÐÅÏ¢£¬ÏÞÖÆÐÔÄÚÇÐøµÄÖÖÀàÐÅÏ¢£»

ÑùÆ·×ÜÁ¿£ºÃ¿¸öÑùÆ·ÖƱ¸ÐèÒª´óÓÚ3 ¦Ìg µÄÑùÆ·£¬Îª°ü¹ÜʵÑéÖÊÁ¿¼°ÑÓÐøÐÔ£¬ÇëÒ»´ÎÐÔÌṩÖÁÉÙ4 ¦Ìg µÄÑùÆ·£»

ÑùƷŨ¶ÈºÍ´¿¶È£ºÑùƷŨ¶È>50 ng/¦Ìl£¬OD260/280 ½éÓÚ1.8-2.0 Ö®¼ä£¬ÎÞÂÑ°×ÖÊ¡¢RNA »òÈâÑۿɼûÔÓÖÊÎÛȾ£»

ÑùÆ·ÖÊÁ¿£º»ùÒò×éÍêÕû¡¢ÎÞ½µ½â£¬µçӾЧ¹û»ùÒò×éDNA Ö÷´øÓ¦ÔÚ-Hind III digest ×î´óÌõ´ø23 kb ÒÔÉÏÇÒÖ÷´øÇåÎú£¬ÎÞÃÖÉ¢£»

ÑùÆ·ÉúÑÄ£ºÇëÑ¡Ôñ¸É·Û¡¢¾Æ¾«¡¢TE buffer »ò³¬´¿Ë®Ò»ÖÖ£¬²¢ÔÚÑùÆ·ÐÅÏ¢µ¥ÖÐ×¢Ã÷¡£

 

ϸ°ûÆ÷»ùÒò×éde novo ²âÐò

 

ÑùÆ·×ÜÁ¿£ºÊ¹ÓÃÃܶÈÌݶÈÀëÐÄÊèÉ¢ºóµÄÑùÆ·Ò»´ÎÖƱ¸DNA ÎÄ¿âµÄÁ¿ÖÁÉÙΪ0.5 ¦Ìg DNA£¬PCR ¸»¼¯ÒªÁìÑùÆ·Ò»´ÎÖƱ¸ÐèÒªÖÁÉÙ

200 ng DNA£¬½¨Òé¿Í»§ÌṩÁ½´Î¼ÆÆ·µÄ×ÜÁ¿£»

ÑùƷŨ¶ÈºÍ´¿¶È£ºOD260/280 ½éÓÚ1.8-2.0 Ö®¼ä£¬ÎÞÂÑ°×ÖÊ¡¢RNA »òÈâÑۿɼûÔÓÖÊÎÛȾ£»

ÑùÆ·ÖÊÁ¿£º»ùÒò×éÍêÕû¡¢ÎÞ½µ½â£¬µçӾЧ¹û»ùÒò×éDNA Ö÷´øÇåÎú£¬ÎÞÃÖÉ¢£»

ÑùÆ·ÉúÑÄ£ºÇëÑ¡Ôñ¸É·Û¡¢¾Æ¾«¡¢TE buffer »ò³¬´¿Ë®Ò»ÖÖ£¬²¢ÔÚÑùÆ·ÐÅÏ¢µ¥ÖÐ×¢Ã÷¡£

 

À©Ôö×Ó²âÐò

 

³£¼ûÇéÐÎÑù±¾£¨ÇëʹÓøɱù»ò±ù´üÔËËÍ£©£ºÍÁÈÀ¡¢ÓÙÄà¡¢³Á»ýÎï ¡Ý 3 g£¬·à±ã ¡Ý 500 mg£¬×éÖ¯Ñù±¾ ¡Ý 1g£»

Ë®ÌåËÍÑùΪ¹ýÂ˺óµÄÂËĤ( ×îÊÊÂËĤֱ¾¶3-4 cm£¬¿×¾¶¾ÞϸһÑùƽ³£Ñ¡Ôñ0.22 ¦Ìm »òÕß0.45 ¦Ìm)£»

ÊÃ×ÓÑù±¾ ¡Ý 2 ¸ö£»

DNA/PCR ²úÆ·£¨ÇëʹÓøɱù»ò±ù´üÔËËÍ£©£»

DNA£ºÅ¨¶È ¡Ý 5 ng/¦Ìl£¬×ÜÁ¿ ¡Ý 150 ng£¬DNA ÒªÓÐÏÔ×ÅÖ÷´ø£¬ÎÞ½µ½â£¬ÎÞRNA¡¢ÂÑ°×ÖʵÈÔÓÖÊÎÛȾ£»

PCR ²úÆ·£ºÅ¨¶È ¡Ý 20 ng/¦Ìl£¬×ÜÁ¿ ¡Ý 400 ng£¬Æ¬¶Ï´óС£¼ 450 bp£¬Ìõ´ø¼òµ¥£¬ÎÞ½µ½â£¬ÎÞÒýÎï¶þ¾ÛÌå¡£




020-89053723
bet356ÌåÓýÔÚÏß
ÓÊÏ䣺ige_info@163.com
QQ£º1787730693
µØµã£º¹ãÖÝÊлÆÆÒÇø¿Æѧ³Ç¿Æ·á·31ºÅ»ªÄÏÐÂÖÊÁÏÁ¢ÒìÔ°G1¶°7Â¥

bet356ÌåÓýÔÚÏß(ÑÇÖÞ°æ)¹Ù·½ÍøÕ¾-App Store

°æȨËùÓÐ ? bet356ÌåÓýÔÚÏß ÔÁICP±¸2021146096ºÅ
EN

×ÉѯÔÚÏß¿Í·þ

bet356ÌåÓýÔÚÏß(ÑÇÖÞ°æ)¹Ù·½ÍøÕ¾-App Store

΢ÐÅЧÀͺÅ